Joint CMU-Pitt Ph.D. Program in Computational Biology and Department of Computational and Systems Biology

  • Assistant Professor
  • Departments of Chemsitry and Molecular Biophysics and Biochemistry
  • Yale University

Discovery and Characterization of Microproteins

Recent advances in next-generation sequencing and proteogenomics have revealed thousands of microprotein-encoding small open reading frames in both prokaryotic and eukaryotic genomes, but we are only just beginning to understand their functions. We describe new quantitative and enrichment-based proteomic methods to reveal regulated, stress-responsive translation of unannotated microproteins, as well as molecular validation of non-AUG start codons from novel genes deriving from both prokaryotes and eukaryotes. Finally, we describe functional characterization of the human microprotein NoBody, which inhibits mRNA decay via association with RNA-protein granules called P-bodies and, more specifically, with the protein EDC4. Taken together, our results suggest that quantitative proteomics and molecular characterization can combine to reveal the functions of novel microproteins.

About the Speaker.

Faculty Host: Anne-Ruxandra Carvunis

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