Budding index results
To validate the accuracy of our synchronization loss model we generated
new budding index data.
Yeast cells (W303 strain Z1321) were grown to OD600 of 0.2 in YPD.
The cells were synchronized by adding alpha factor (5ug/ml) to the growth
medium. After 2 hours incubation the culture were completely arrested in G1
(all the cell were without buds). The synchronization was released by washing
out the alpha factor from the medium (by pelleting the cells and changing to
a fresh medium) and the cells were grown for additional 90 minutes.
Samples were taken every 15 minutes, fixed (1\% formaldehyde) and observed
under a light microscope. For each time point 200 cells were counted and the
fraction of cells with no bud, a small bud (smaller than one half of the
yeast cell) or a large bud (equal or larger than one half of the yeast cell)
was documented. You can download an Excel sheet with the results
from here.